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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Comparative vascular responses three months after paclitaxel and everolimus-eluting stent implantation in streptozotocin-induced diabetic porcine coronary arteries

Figure 3

Effects of paclitaxel and everolimus on SMC and EC apoptosis and necrosis. SMC or EC were cultured in vitro under hyperglycemic conditions (42 mM) in 24-well plates and allowed to grow to 80% confluence. Cells were then treated with growth media alone (control), DMSO vehicle control, or everolimus or paclitaxel over a range of concentrations (10-5 M–10-12 M) for 24 hours. A Cell Death Detection ELISAPLUS kit was then used to detect histone-associated nucleosome concentration in cell media supernatants to determine overall necrosis, while concentration in cell lysates was used to determine relative induction of apoptosis. Data expressed as fold change relative to control (mean ± standard deviation, n = 3). A) Paclitaxel significantly increased SMC apoptosis and necrosis in a dose-dependent manner at concentrations of 1 μM and greater. B) Paclitaxel significantly increased EC apoptosis and necrosis in a dose-dependent manner at concentrations of 0.1 μM and greater. Everolimus did not increase SMC or EC apoptosis or necrosis at any dose tested. * p < 0.05 vs. control

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