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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Fatty acid-binding protein 4 impairs the insulin-dependent nitric oxide pathway in vascular endothelial cells

Figure 1

Effect of FABP4 on eNOS activation and NO production in HUVECs. A, effect of FABP4 on eNOS phosphorylation at Ser1177. HUVECs were incubated with FABP4 at the indicated concentrations for 30 minutes. B, eNOS Ser1177 phosphorylation in response to various doses of insulin (100, 300, 600 nM). HUVECs were incubated with insulin at the indicated concentrations for 30 minutes. C, Time course of eNOS Ser1177 phosphorylation in response to insulin. HUVECs were incubated with 600 nM insulin for 10, 30 and 60 min. D, effect on insulin-stimulated eNOS phosphorylation at Ser1177. HUVECs were incubated with FABP4 at the indicated concentrations for 30 minutes and then with insulin (600 nM) for 30 minutes. E, effect of FABP4 on NO production. HUVECs were incubated with FABP4 at indicated concentrations for 30 minutes and then stimulated with insulin (600 nM for 30 min). Representative blots are shown. The data are given as the mean ± standard deviation from three independent experiments. *P < 0.05 vs. insulin(−) / FABP4(−); #P < 0.05 vs. insulin(+) / FABP4(−).

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