Effect of RhoA N19 on moesin phosphorylation induced by AGE-HSA. RhoA N19 was transfected to HMVECs 24 h before AGE-HSA (50 mg/L, 60 min) stimulation and moesin phosphorylation was detected by immunoblotting. RhoA L63 was transfected to HMVECs for 24 h as a positive control and phosphor-moesin was assayed. Culture medium was used as blank control. Results shown are representative IB (A) and densitometry results (B). n = 3 independent experiments. *P < 0.05, vs control, #P < 0.05 vs AGE-HSA.