Figure 5
From: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response
Effect of transfection of RhoA N19 or RhoA L63 on RhoA activity and ROCK phosphorylation. At 24 h after transfection, HMVECs were treated with 50 mg/L AGE-HSA for 60 min. RhoA activity and ROCK phosphorylation were assessed with a luminescence-based G-LISA™ assay and immunoblotting, respectively. Culture medium was used as control. *P < 0.05, **P < 0.01 vs control, #P < 0.05 vs AGE-HSA.