Figure 4
From: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response
AGE-HSA increased phosphorylation of ROCK in a time- and dose-dependent manner. HMVECs were treated with 50 mg/L AGE-HSA for 30, 45, 60, 90, or 120 min (A), or with 12.5, 25, 50, or 100 mg/L AGE-HSA for 120 min (B). Phosphorylation of ROCK was detected by immunoblotting. Culture medium was used as blank control and HSA was used as albumin control. Results shown are representative IB (A and B) and densitometry results (C and D). n = 3 independent experiments, *P < 0.05, **P < 0.01 vs control.