Figure 1
From: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response
AGE-HSA induced time-(A) and dose-(B) dependent increases of HMVEC monolayer permeability. HMVECs were treated with 50 mg/L AGE-HSA for 1, 2, 4, 8, or 12 h, or with 12.5, 25, 50, or 100 mg/L AGE-HSA for 8 h. Culture medium was used as the blank control and HSA was used as the albumin control. Permeability was measured from the transflux of tracer protein TRITC-albumin across monolayers and was expressed as a coefficient for albumin (Pa). n = 3, *P < 0.05, **P < 0.01 vs control.