Figure 4

Effect of fasudil on HG-induced Rho/rock activation. A: Confluent HUVECs were stimulated with HG or HG (20 mM) + fasudil (10^-5 mM) for different incubation times (0, 15 min, 30 min, 1 h, 3 h and 6 h). Protein was determined by Western blot analysis. Each band density was normalized by its own ratio of p-MYPT/MYPT and results were expressed as fold induction respect to time 0. B: Means ± SD of at least three experiments was shown. * P < 0.05, when compared to time 0. # P < 0.05, when compared to HG alone.