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Figure 1 | Cardiovascular Diabetology

Figure 1

From: GLUT4, GLUT1, and GLUT8 are the dominant GLUT transcripts expressed in the murine left ventricle

Figure 1

Detection of GLUT mRNAs in the left ventricle of C57BL/6J mice. A) PCR was employed to identify each known murine member of the facilitative glucose transporter family in the left ventricle of 10-week-old male C57BL/6J mice. GLUTs-1, -3, -4, -8, -10, and −12 were amplified using cDNA derived from the left ventricular myocardium. The identity of each GLUT amplicon was confirmed by sequencing. GLUTs-2, -5, -6, and −9 were not present in the left ventricle of male C57BL/6J mice. Representative images are shown (n = 3). B) The specificity of the primers used to amplify GLUTs-2, -5, -6, and −9 were confirmed using cDNA derived from the positive control tissues: liver, intestine, and brain. The specificity of the primers for each GLUT amplicon was confirmed by sequencing.

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