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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Increased myocardial SERCA expression in early type 2 diabetes mellitus is insulin dependent: In vivo and in vitro data

Figure 4

In vitro data: Effect of insulin on SERCA2a/PLB mRNA ratio (A) and (B) EHT relaxation data (T2). EHT were stimulated for 5 days with 0.10 μg/mL insulin over 5 days (n = 7-10) in each group from two independent cell preparations. Transcript concentration was determined by mRNA was measured by quantitative RT-PCR. *p < 0.01 vs. EHT. (C) Correlation between relaxation time and SERCA2a/PLB ratio in EHT´s without and with prior stimulation of insulin. Contractile response of isoprenaline (1 μmol/L) was determined under isometric conditions and in the presence of 0.2 mmol/L calcium (n = 7-8 in each group from two independent cell preparations). For clarity, only mean values ± SEM of the two groups are shown. *p < 0.05 vs. non insulin treated EHTs. In (B) relaxation time (T2) was significantly shorter in insulin stimulated EHTs (p < 0.05).

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