Figure 2
Effect of high-fat diet in the generation of intraplatelet reactive-oxygen species (ROS). Male Wistar rats were fed with either a standard chow diet (SCD) or high-fat diet (HFD) during 10 weeks. Washed platelets (1.2 × 108 platelets/ml) from SCD or HFF rats were pre-incubated with N-acetylcysteine (NAC, 1 mM for 15 min) or PEG-catalase (1000 mU/ml, 15 min) and then stimulated with ADP (10 μM). Generation of ROS was quantified by flow cytometry using 2'-7'-dichloroflurescin diacetate (DCFH-DA). Results are shown as mean ± SEM values for n = 6-7. *P < 0.05 compared with SCD group. #P < 0.05 compared with ADP in HFF group. MFI = mean fluorescence index.