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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Advanced glycation end products impair the migration, adhesion and secretion potentials of late endothelial progenitor cells

Figure 1

Characterization of EPCs derived from bone marrow. A: MNCs were isolated and plated on fibronectin-coated culture dish on the first day (100×). B: Seven days after plating, adherent early EPCs with spindle shape formed clones (100×). C: After 3~4 weeks, the third-fifth passage cells (late EPCs) showed cobblestone-like morphology (100×). D-F: Most early EPCs were shown to simultaneously endocytose DiI-acLDL (red) and bind fluorescein isothiocyanate UEA-1 (lectin) (100×). G: FACS analysis showing the expressions of CD133 and Sca-1 in early EPCs. Plots show isotype controls (black) vs. specific antibody staining (red). H: FACS analysis showing the phenotype of late EPCs using several markers: vWF, VEGFR-2, VE-cadherin and PECAM-1. Plots show isotype controls (black) vs specific antibody staining (red). I-J: Representative images of tubuli like structures formed on Matrigel by early and late EPCs(100×).

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