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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Effects of some anti-diabetic and cardioprotective agents on proliferation and apoptosis of human coronary artery endothelial cells

Figure 4

Insulin, metformin, BLX-1002, pioglitazone and candesartan protect HCAECs against palmitate-induced caspase-3 activation. Palmitate-induced caspase-3 activity, a measure of apoptosis, was evaluated using the EnzChek® Caspase-3 Assay Kit. HCAECs were incubated in medium containing 5 mM glucose, supplemented with 0.5% FBS, with or without insulin lispro (1 nM), BLX-1002 (1 μM), metformin (500 μM), pioglitazone (2.5 μM), candesartan (100 nM) in the presence of 0.125 mM palmitate or vehicle (ethanol and BSA) for 24 h. * denotes P < 0.05 for a chance difference vs. controls by ANOVA. # denotes P < 0.05 for a chance difference vs. palmitate by ANOVA.

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