Table 1 The sequences of primers used in this experiment
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
GAPDH | GCAAGTTCAACGGCACAG | GCCAGTAGACTCCACGACAT |
Hu-StAR | CTGAGGCAACAGGCTGTGAT | AGCCGAGAACCGAGTAGAGAG |
Rat-StAR | CACAGTCATCACCCATGAGC | AGCTCTGATGACACCGCTTT |
ACC-1 | CCCAACAGAATAAAGCTACTCTGG | TCCTTTTGTGCAACTAGGAACGT |
FAS | CCTCTTCCCTGGCACTGGCTACCT | ACTCGGCGGGGATCGGGACTT |
LDLR | AAGCCATTTTCAGTGCCAAC | AGGTGAACTTGGGTGAGTGG |
HMGR | TGCTGCTTTGGCTGTATGTC | TGAGCGTGAACAAGAACCAG |
SREBP-2 | CATCTTCCCCTCTCCTTCCTAT | CCCAGCTTGACAATCATCTGC |
IL-1β | CTGTGACTCGTGGGATGATG | GGGATTTTGTCGTTGCTTGT |
TNFα | GCTACGGGCTTGTCACTC | CCACGCTCTTCTGTCTACTG |
VCAM-1 | ACAAAACGCTCGCTCAGATT | GTCCATGGTCAGAACGGACT |
IL-6 | CACAAGTCCGGAGAGGAGAC | ACAGTGCATCATCGCTGTTC |