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Figure 6 | Cardiovascular Diabetology

Figure 6

From: Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability

Figure 6

Cerulenin and lovastatin has the similar effect while StAR siRNA has the reverse effect as StAR overexpression in inflammatory response and NO bioavailability. Before treated with PA (200 μM), RAECs were treated with 5 μg/ml cerulenin (in ethonal), or 5 μM lovastatin (in DMSO), or 3.3 μg/ml cerulenin plus 3.3 μM lovastatin for 24 hours. In another experiment, RAECs were transfected with 100 nM siRNA specific for StAR for 24 hours. The mRNA levels of IL-1β, TNFα, VCAM-1 and IL-6 were detected by real-time quantitative PCR and NO content was detected by Nitric Oxide Detection Kit after PA treatment for 2 hours. A. The mRNA levels of IL-1β, TNFα, VCAM-1, IL-6 were analyzed by real-time quantitative PCR in cells pre-treated with indicial inhibitor. B. The mRNA levels of IL-1β, TNFα, VCAM-1, IL-6 were analyzed by real-time quantitative PCR in cells pre-treated with siRNA specific for StAR. Inset shows the confirmation of StAR mRNA reduction with siRNA transfection by real-time quantitative PCR. Data represented as a ratio of gene-to-GAPDH expression (relative mRNA levels) and ratio to the control treatment mRNA levels at any indicial time. Data represents the means ± S.D. from three independent experiments. C. NO contents in the culture supernatant. Data represents the mean ± SE from three independent experiments. * represents P < 0.05.

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Cardiovascular Diabetology

ISSN: 1475-2840