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Figure 5 | Cardiovascular Diabetology

Figure 5

From: Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability

Figure 5

StAR overexpression attenuated PA-induced reduction in NO bioavailability. RAECs were treated with PA (200 μM) in a time-dependent manner after infected with adenovirus as indicated. The protein levels of phosphor-Akt Ser 473, total Akt, phosphor-eNOS Ser 1177 and total eNOS were detected by Western blot. The NO content in media supernatant was detected by Nitric Oxide Detection Kit. A: Western blotting analysis of phosphor-Akt Ser 473 and total Akt. B: Western blotting analysis of phosphor-eNOS Ser 1177 and total eNOS. β-actin was used as the loading control. Data represent the means ± S.D. from three independent experiments. C: NO contents in the culture supernatant. Data represents the mean ± SE from three independent experiments. * represents P < 0.05.

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