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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability

Figure 1

StAR overexpression in RAECs by adenovirus infection. Primary cultured RAECs were infected with the indicated recombinant adenoviruses as described in the “Methods” section. StAR mRNA and protein levels were determined by real-time Quantitative PCR (A) and Western blotting analysis (B). A: Real-time quantitative PCR analysis of StAR mRNA levels with adenovirus infection at different MOI (MOI = 10, 20, 50, 100) for 48 h. The data was normalized to internal GAPDH mRNA expression and presented as fold change. B: Western blotting analysis of StAR protein levels in RAECs after infection. β–actin serves as the loading control and a representative image is shown in upper panel. The data represent the means ± S.D. from three independent experiments and shown as fold change over the cells treated with Ad-CMV-EGFP. EGFP represents cells infected with recombinant adenovirus encoding CMV-EGFP and served as the negative control; StAR, cells infected with adenovirus encoding CMV-StAR; * represents P < 0.05.

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Cardiovascular Diabetology

ISSN: 1475-2840