Regional evidence of modulation of cardiac adiponectin level in dilated cardiomyopathy: pilot study in a porcine animal model

Table 1 Sequence of RT-PCR primer pairs for analyzed genes

Primer	GenBank	Sequence	Ta, °C	Cycles, n
ADN	EF601160.1	Forward: ATCTGGAGTGACTGGGGTTG	61	36
202 bp	EF601160.1	Reverse: TTTGCCAGTGGTGACATCAT	61	36
AdipoR1	AB527058.1	Forward: AACCCACCCAAAGCTGAAGA	58	29
334 bp	AB527058.1	Reverse: CTGAGCATGGTCAAGATTCC	58	29
AdipoR2	NM_001007192	Forward: GCCTGGGGATCTTTTATATGTTTC	53	34
650 bp	NM_001007192	Reverse: GCCGATCATGAAACGAA	53	34
T-CAD	NM_001109945.1	Forward: CCCGGGCAGAGCTTCGAAAT	64	25
217 bp	NM_001109945.1	Reverse: GGTAGAAAACCGGGCCCTCG	64	25
PPAR-α	NM_001044526.1	Forward: TCGCGGGAAAGGCCAGCAAT	70	27
431 bp	NM_001044526.1	Reverse: GTCGTCCAGCTCCAGCGCATT	70	27
PPAR-γ	DQ437884.1	Forward: AGGAGCATAGCAAAGAGGTGGCC	70	32
391 bp	DQ437884.1	Reverse: AGCAAACCTGGGCGGTCTCCA	70	32
BNP	M25547	Forward: GTGCTCCTGCTCCTGTTCTT	61	35
451 bp	M25547	Reverse: TCCCAGGCTTCTGTGAGG	61	35
iNOS	NM_001143690.1	Forward: GAGGGCAGCCAAGGCCCAAG	65	35
264 bp	NM_001143690.1	Reverse: GGCCAGCTTTCGGAGCAGCA	65	35
TNF-α	EU682384.1	Forward: ATCGGCCCCCAGAAGGAAGAC	64	33
351 bp	EU682384.1	Reverse: GATGGCAGAGAGGAGGTTGAC	64	33
GAPDH	AF017079	Forward: ACCACAGTCCATGCCATCAC	60	30
451 bp	AF017079	Reverse: TCCACCACCCTGTTGCTGTA	60	30
HPRT1	DQ178126	Forward: CCGAGGATTTGGAAAAGGT	60	30
181 bp	DQ178126	Reverse: CTATTTCTGTTCAGTGCTTTGATGT	60	30
TBP	DQ178129	Forward: GATGGACGTTCGGTTTAGG	60	30
124 bp	DQ178129	Reverse: AGCAGCACAGTACGAGCAA	60	30

Adiponectin (ADN), AdipoR1, AdipoR2, T-cadherin (T-CAD), peroxisome proliferator-activated receptor (PPAR)-α, PPAR-γ, brain natriuretic peptides (BNP), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT-1) and TATA binding protein (TBP).

ISSN: 1475-2840