Effects of telmisartan on mRNA expression and adipocyte size distribution in db/db mice fed a high-fat diet. At sacrificed after 5-week treatment, Sirt1, PGC1α, and GLUT4 mRNA expression were semi-quantified by RT-PCR in skeletal muscle and in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). GAPDH was used as an internal control. Distribution of adipocyte size was determined by the Coulter Multisizer III (Beckman Coulter, High Wycombe, England) after fixed with 2% osmium tetroxide. Values shown represent means ± S.E (n = 3-4). *p < 0.05 vs. wild type and †p < 0.05 vs. CMC by Fisher’s PLSD.