Representative WB analyses of pAMPK in H9c2 cells treated with metformin or obestatin (A), in left ventricle of rat subjected to acute treatment with metformin or OB (B) and in left ventricle of non-diabetic (ND) and diabetic rats (DM), treated or not with OB for 6 weeks (C). Representative WB analyses of pGSK3β (D) in left ventricle of non-diabetic (ND) and diabetic rats (DM), treated or not with OB for 6 weeks. Quantitative analyses are reported in the histograms, which represent the net intensity ratio of bands with total AMPK or β-actin. Data are expressed as percentage variations from the ND-CTRL value. Data are means ± SD of 8–10 rats per group. Statistical significance: * P < 0.05 vs ND-CTRL; ** P < 0.01 vs ND-CTRL; *** P < 0.005 vs ND-CTRL; † P < 0.05 vs DM-CTRL; †† P < 0.01 vs DM-CTRL.