Representative semiquantitative RT-PCR analyses of CTGF and TGF-β1 in left ventricle RNA extracts from non-diabetic (ND) and diabetic rats (DM), treated or not with OB. Quantitative analyses are reported in the histogram, which represents the ratio of net intensity of bands with GAPDH. Data are expressed as % variations from the ND-CTRL value. Data are means ± SD of 8–10 rats per group. Statistical significance: * P < 0.05 vs ND-CTRL; ** P < 0.01 vs ND-CTRL; † P < 0.05 DM-CTRL.