Representative TNFα plasma concentration determined by ELISA (A). Representative WB analyses of NFkB-p65 in cytosol (B) and in nucleus (C) obtained from left ventricular tissue from non-diabetic (ND) and diabetic rats (DM), treated or not with OB. Quantitative analyses are reported in the histograms, which represent the net intensity ratio of bands with α-actin or lamin-B1. Data are expressed as % variations from the ND-CTRL value. Data are means ± SD of 8–10 rats per group. Statistical significance: * P < 0.05 vs ND-CTRL; ** P < 0.01 vs ND-CTRL; † P < 0.05 vs DM-CTRL.