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Table 1 The sequences of forward (F) and reverse (R) primers and restriction enzymes for genotyping of the ten SNPs

From: Several genetic polymorphisms interact with overweight/obesity to influence serum lipid levels

SNP Primer sequence Restriction enzyme PCR product Allele
ABCA-1 V825I F: 5′-GGTAGCCCACCACTCTCCCCTATAAAG-3′ Tag I 525 bp G
(rs2066715) R: 5′-ATCAGCTGCCTGTCCTTGGACTA-3′    A
ACAT-1 F: 5′-TATATTAAGGGGATCAGAAGT-3′ Rsa I 389 bp A
(rs1044925) R: 5′-CCACCTAAAAACATACTACC-3′    C
LDL-R Ava II F: 5′-GTCATCTTCCTTGCTGCCTGTTTAG-3′ Ava II 228 bp A-
  R: 5′-GTTTCCACAAGGAGGTTTCAAGGTT-3′    A+
LIPC -250G>A F: 5′-GGCAAGGGCATCTTTGCTTC-3′ Dra I 411-bp G
(rs2070895) R: 5′-GGTCGATTTACAGAAGTGCTTC-3′    A
LIPG 584C>T F: 5′-CATGAGCTGAGATTGTTGTCAGTGC-3′ Nde I 254 bp C
(rs2000813) R: 5′-CAGTCAACCACAACTACATTGGCGTCTTTCTCTCAT-3′    T
MTHFR 677C>T F: 5′-CAAAGGCCACCCCGAAGC-3′ Hinf I 254 bp C
(rs1801133) R: 5′-AGGACGGTGCGGTGAGAGTG-3′    T
MYLIP F: 5′-ACAGAGCAAAAGACCCTGTCTC-3′ Hae III 387 bp G
(rs3757354) R: 5′-AAAGAACTGTGTGTGGGAGGAT-3′    T
PCSK9 E670G F: 5′-CACGGTTGTGTCCCAAATGG-3′ Eam 1104I 440 bp A
(rs505151) R: 5′-GAGAGGGACAAGTCGGAACC-3′    G
PPARD +294T>C F: 5′-CATGGTATAGCACTGCAGGAA-3′ Bsl I 269 bp T
(rs2016520) R: 5′-CTTCCTCCTGTGGCTGCTC-3′    C
SCARB1 F: 5′-CCTTGTTTCTCTCCCATCCTCACTTCCTCGACGC-3′ Hin I1 218 bp C
(rs5888) R: 5′-CACCACCCCAGCCCACAGCAGC-3′    T