Table 1 The sequences of forward (F) and reverse (R) primers and restriction enzymes for genotyping of the ten SNPs
From: Several genetic polymorphisms interact with overweight/obesity to influence serum lipid levels
SNP | Primer sequence | Restriction enzyme | PCR product | Allele |
|---|---|---|---|---|
ABCA-1 V825I | F: 5′-GGTAGCCCACCACTCTCCCCTATAAAG-3′ | Tag I | 525 bp | G |
(rs2066715) | R: 5′-ATCAGCTGCCTGTCCTTGGACTA-3′ |  |  | A |
ACAT-1 | F: 5′-TATATTAAGGGGATCAGAAGT-3′ | Rsa I | 389 bp | A |
(rs1044925) | R: 5′-CCACCTAAAAACATACTACC-3′ |  |  | C |
LDL-R Ava II | F: 5′-GTCATCTTCCTTGCTGCCTGTTTAG-3′ | Ava II | 228 bp | A- |
|  | R: 5′-GTTTCCACAAGGAGGTTTCAAGGTT-3′ |  |  | A+ |
LIPC -250G>A | F: 5′-GGCAAGGGCATCTTTGCTTC-3′ | Dra I | 411-bp | G |
(rs2070895) | R: 5′-GGTCGATTTACAGAAGTGCTTC-3′ |  |  | A |
LIPG 584C>T | F: 5′-CATGAGCTGAGATTGTTGTCAGTGC-3′ | Nde I | 254 bp | C |
(rs2000813) | R: 5′-CAGTCAACCACAACTACATTGGCGTCTTTCTCTCAT-3′ |  |  | T |
MTHFR 677C>T | F: 5′-CAAAGGCCACCCCGAAGC-3′ | Hinf I | 254 bp | C |
(rs1801133) | R: 5′-AGGACGGTGCGGTGAGAGTG-3′ |  |  | T |
MYLIP | F: 5′-ACAGAGCAAAAGACCCTGTCTC-3′ | Hae III | 387 bp | G |
(rs3757354) | R: 5′-AAAGAACTGTGTGTGGGAGGAT-3′ |  |  | T |
PCSK9 E670G | F: 5′-CACGGTTGTGTCCCAAATGG-3′ | Eam 1104I | 440 bp | A |
(rs505151) | R: 5′-GAGAGGGACAAGTCGGAACC-3′ |  |  | G |
PPARD +294T>C | F: 5′-CATGGTATAGCACTGCAGGAA-3′ | Bsl I | 269 bp | T |
(rs2016520) | R: 5′-CTTCCTCCTGTGGCTGCTC-3′ |  |  | C |
SCARB1 | F: 5′-CCTTGTTTCTCTCCCATCCTCACTTCCTCGACGC-3′ | Hin I1 | 218 bp | C |
(rs5888) | R: 5′-CACCACCCCAGCCCACAGCAGC-3′ |  |  | T |