Effect of ALA on cardiac HO-1 and Cu/Zn-SOD expression in OLETF rats. (A) Western blot showing the level of cardiac HO-1 in each group. Quantification of cardiac HO-1 by Western blot analysis. (B) Western blot showing the level of cardiac Cu/Zn-SOD in each group. Quantification of cardiac Cu/Zn-SOD by Western blot analysis. The densitometry value of each protein was normalized to that of α-tubulin and the values are presented as arbitrary units (A.U.) relative to LETO expression levels. Data are presented as the mean ± SEM. *p < 0.05 vs. LETO rats; †p < 0.05 vs. OLETF rats. (C) Representative micrographs of immunostained cardiac Cu/Zn-SOD in each group. Scale bar = 100 μm.