Figure 4

Total GLUT4 protein in heart (A), gastrocnemius muscle (B) and epididymal white adipose tissue (C) from the animals studied. C: Wistar-Kyoto rats that did not receive any treatment; H: spontaneously hypertensive rats that did not receive any treatment; MetS: spontaneously hypertensive rats that received MSG during the neonatal period. On the top, representative images of GLUT4 and respective loading controls. Loading controls are β-actin for white adipose tissue, and total proteins of 35 to 130 kDa range of the Ponceau stained membrane for heart and gastrocnemius, as described in Methods. On the bottom, the graphs show means ± SEM of n = 5 animals. Two-way analysis of variance (ANOVA) Panel A: group, time and interaction (p<0.001); Panel B: group (p = 0.006), time (p<0.001) and interaction (p = 0.003); Panel C: group, time and interaction (p<0.001), followed by the Bonferroni’s post hoc test: *** p<0.001 vs. C; † p<0.05 and ††† p<0.001 vs. H at same time. The time course changes inside groups are also showed.