Eplerenone prevents salt-induced vascular stiffness in Zucker diabetic fatty rats: a preliminary report

Table 2 Structural, histological and immunohistochemical characteristics of mesenteric resistance arteries:

	ZL	ZL+S	ZDF	ZDF+E	ZDF+S	ZDF+S+E	ZDF+S+H	P-value
Media (μm)	17.9 ± 0.9	22.2 ± 1.9	19.8 ± 0.9	21.1 ± 1.1	28.7 ± 1.8◆	22.6 ± 2.0	23.6 ± 1.2	< 0.001
Lumen (μm)	297 ± 14	306 ± 22	298 ± 11	301 ± 21	273 ± 12	305 ± 13	302 ± 17	0.82
Media/Lumen (%)	6.1 ± 0.3	7.3 ± 0.3	6.7 ± 0.3	7.1 ± 0.3	10.7 ± 0.8♠	7.5 ± 0.7	7.9 ± 0.4	< 0.001
Exponent Strain/stress relation	6.4 ± 0.4	6.3 ± 0.3	6.5 ± 0.5	5.7 ± 0.3	7.5 ± 0.6	5.6 ± 0.3	6.4 ± 0.7	0.09
Collagen IV (%)	17.1 ± 0.7	25.9 ± 0.4*	20.9 ± 0.7§	18.3 ± 0.4¥¥	27.0 ± 0.4¥	21.3 ± 0.4¡	21.5 ± 0.5¡ ¡	< 0.001
Elastin (%)	21.6 ± 1.3	15.6 ± 1.2	18.8 ± 1.2	23.5 ± 1.4	17.6 ± 2.0	18.0 ± 1.2	19.2 ± 0.6	0.01
Sirius red (%)	12.1 ± 0.4	19.0 ± 0.9	16.2 ± 0.6	17.2 ± 0.6	19.6 ± 0.6♤	17.5 ± 0.9	17.1 ± 0.7✚	< 0.001

Abbreviations: ZL: Zucker lean on normal diet. ZL + S: Zucker lean on high salt diet. ZDF: Zucker diabetic fatty. ZDF + E: Zucker diabetic fatty on eplerenone therapy. ZDF + S: Zucker diabetic fatty on high salt diet. ZDF + S + E: Zucker diabetic fatty on high salt diet with eplerenone therapy. ZDF + S + H: Zucker diabetic fatty on high salt diet with hydralazine therapy.
Post hoc testing: * P < 0.001 ZL vs. ZL+S. § P < 0.001 ZL vs. ZDF. ¥ P < 0.001 ZDF vs. ZDF+S. ¥¥ p < 0.001 ZDF vs. ZDF+E. ¡ P < 0.001 ZDF+S vs. ZDF+S+E. ¡ ¡ p < 0.001 ZDF+S vs. ZDF+S+H. ◆ P < 0.05 ZDF+S vs. all other groups. ♠ P < 0.001 ZDF+S vs. all other groups. ♤ P < 0.05 ZDF vs. ZDF + S. ✚ p < 0.05 ZDF + S vs. ZDF + S + H.

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