Figure 4

The representative sections of immunohistochemical staining for TGF-β in non-treated and G-CSF treated OLETF rats. Tissues expressing TGF-β appeared brown color. (*200). The minimal interstitial staining for TGF-β is found in OLETF (-/-), whereas complete absence in OLETF (-/+). Similarly, the extent of TGF-β immunoreactivity in the interstitial and perivascular tissue is significantly decreased in OLETF (+/+) compared with OLETF (+/-).A. OLETF (-/-); OLETF rat fed on tap water and treated with saline, B. OLETF (-/+); OLETF rat fed on tap water and treated with G-CSF, C. OLETF (+/-); OLETF rat fed on sucrose water and treated with saline, D. OLETF (+/+); OLETF rat fed on sucrose water and treated with G-CSF.