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Table 2 Pharmacological parameters of vasomotor responses to phenilephrine ( PE), acetylcholine ( Ach), sodium nitroprusside ( SNP) and asymmetric NG, NG-dimethyl-L-arginine ( ADMA) in isolated aorta

From: Effect of silibinin on endothelial dysfunction and ADMA levels in obese diabetic mice

PE
  pD2 EC50 (nmol/L) Emax (% K+)
db/m + vehicle 7.27 ± 0.11 53.7 (32.1-89.7) 126.0 ± 7.0
db/db + vehicle 7.74 ± 0.10** 18.3 (11.3-29.9) 122.3 ± 5.0
db/db + silibinin 7.66 ± 0.08** 21.7 (15.3-30.7) 122.6 ± 3.7
ADMA
  pD 2 EC 50 ( μmol/L) E max (% K + )
db/m + vehicle 3.36 ± 1.76 440 (0.1-106) 65.6 (± 154)
db/db + vehicle 4.48 ± 0.36** 32.8 (5.8-188) 69.6 ± 12.0
db/db + silibinin 4.51 ± 0.40** 30.1 (4.8-194) 71.2 ± 12.6
ACh
  pD 2 EC 50 (nmol/L) E max (% tone)
db/m + vehicle 7.54 ± 0.12 28.8 (16.7-49.5) 35.7 ± 2.5
db/db + vehicle 6.74 ± 0.15** 183 (90.2-372) 62.1 ± 2.1**
db/db + silibinin 6.89 ± 0.12** 130 (74.9-224) 43.7 ± 2.8††
SNP
  pD 2 EC 50 (nmol/L) E max (% tone)
db/m + vehicle 7.97 ± 0.04 10.1 (8.8-12.9) 9.4 ± 1.0
db/db + vehicle 7.45 ± 0.06** 35.7 (26.5-48.0) 16.7 ± 1.7*
db/db + silibinin 7.56 ± 0.05** 27.7 (21.7-35.3) 11.8 ± 1.5
  1. Pharmacological parameters are from non linear regression; pD2 is the negative logarithm of the concentration producing 50% of the maximum effect, EC50 is the concentration producing 50% of maximum effect (95% confidence limits are given in parenthesis), Emax is the maximum effect (vasoconstriction to PE or ADMA in % of vasoconstriction evoked in the same preparations by 100 mmol/L K+; vasodilatation to ACh or SNP as % of residual tone). Statistical comparisons were made on the whole curves by two-way ANOVA; n = 12 preparations from 6 mice in each group; *P < 0.05, **P < 0.01 versus db/m + vehicle, ††P < 0.01 versus db/db + vehicle.