Glucagon-like peptide-1 enhances cardiac L-type Ca2+ currents via activation of the cAMP-dependent protein kinase A pathway

Table 1 Effects of GLP-1 on action potentials of canine left ventricular cardiomyocytes

	MHP mV	AAP mV	APT mV	Vmax V/s	APD₅₀ms	APD₉₀ms
Control	-80.2 ± 1.9	149.8 ± 4.6	-61.0 ± 2.4	126.6 ± 17.6	124.3 ± 24.4	236.1 ± 17.6
GLP-1	-84.2 ± 2.0	145.3 ± 4.5	-61.8 ± 1.6	120.8 ± 22.7	252.1 ± 30.5**	434.8 ± 79.2*
Washout	-82.4 ± 2.3	142.2 ± 5.8	-62.4 ± 0.9	113.1 ± 25.5	120 ± 27..7	302.8 ± 66.9

Values are expressed as mean ± SEM (n = 6). GLP-1 (7-36 amide) at 5 nM was added to the bath solution. The data were collected at the times just before GLP-1 (Control), 10 to 15 min after GLP-1 perfusion (GLP-1), and 10 to 15 min after washout of GLP-1 (Washout). MHP, membrane holding potential before elicitation of an action potential; AAP, amplitude of action potential; APT, threshold of initiation of an action potential; V_max, maximum upstroke velocity of action potential; APD, action potential duration measured at 50% (APD₅₀) and 90% (APD₉₀) of repolarization. *, p < 0.05; **, p < 0.01; versus control and washout.

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