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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Therapeutic potential of N-acetylcysteine as an antiplatelet agent in patients with type-2 diabetes

Figure 3

Effect of in vitro additions of NAC on antioxidant capacity and platelet-derived ROS in platelet samples from patients with type-2 diabetes. (A) Mean data for the effect (n = 12); data are expressed as mean ± sem; statistical analysis was performed by one-way ANOVA with Dunnett's post hoc test vs control. **P < 0.01; ***P < 0.001. (B) Typical 3-line EPR spectra obtained at the 4 h reading for Tyrode's buffer alone and in washed platelets with or without treatment with NAC (10-100 μmol/l; 2 h; 37°C - NAC washed out of supernatant before addition of spin-trap). (C) ROS detection in washed platelets. Luminescence detection of ROS in both the basal state (black bars) and following thrombin-stimulation (white bars) of washed platelets (n = 12). Data are expressed as mean ± sem; statistical analysis was performed by one-way ANOVA with Dunnett's post hoc test vs control for each group (basal and thrombin-activated samples were analysed separately). **P < 0.01; ***P < 0.001 (thrombin-activated vs control): #P < 0.05; ###P < 0.001 (basal vs control). (D) L-NAME sensitive nitrite detection in collagen activated washed platelets (n = 10). Data are expressed as mean ± sem; statistical analysis was performed by one-way ANOVA with Dunnett's post hoc test vs control. **P < 0.01.

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