Expression of glypican-1 in CM (cardiac muscle) and SM (skeletal muscle). A. Typical Western blot of 7.5% SDS-PAGE using antiglypican antibodies in extracts of muscles of control, 24-hour, 10-day, and 30-day induced rats. B. Bars represent the mean values ( ± SE) of arbitrary optical density units (OD) of 4 independent blots (*P < 0.05; ***P < 0.001 compared with control). C and D. Immunohistochemistry from CM and SM for control and 30-day specimens. Arrows indicate the brown-colored precipitates.