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Table 1 Real-time quantitative RT-PCR Primers

From: Decreased adiponectin and increased inflammation expression in epicardial adipose tissue in coronary artery disease

Gene title

Accession number

Primer

Amplicon size (bp)

Adiponectin

XM_290602

Forward 5'-GTCCTAAGGGAGACATGG-3'

302

  

Reverse 5'-GATCTTCATGAGGTAGTCAGT-3'

 

IL-6a

NM_000600

Forward 5'-GTGAAAGCAGCAAAGAGGCA-3'

276

  

Reverse 5'-TTGGGTCAGGGGTGGTTATT-3'

 

TNF-αb

NM_000594

Forward 5'-CCGAGTCTGGGCAGGTCTA-3'

201

  

Reverse 5'-CGAAGTGGTGGTCTTGTTGC-3'

 

TLR4c

NM_138554

Forward 5'-GGCTCACAATCTTATCCAATCT-3'

129

  

Reverse 5'-TGATGTAGAACCCGCAAGTC-3'

 

β-actin

NM_001101

Forward 5'- CCAACCGCFAFAAFATGACC-3'

175

  

Reverse 5'-GATCTTCATGAGGTAGTCAGT-3'

 
  1. a Interleukin-6
  2. b Tumor necrosis factor-α
  3. c Toll like receptor 4
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Cardiovascular Diabetology

ISSN: 1475-2840