Figure 3

Experiment on stimulated monocytes. (A and C) Monocytes were treated with various concentrations of SA, and the histograms showed that obvious dose-effect relationships exist between TNF-α and IL-6 secretion by monocytes and SA; (B and D) Monocytes were treated with various concentrations of gAd along with 0.5 mM SA. The histograms showed that gAd had an inhibitory effect on monocyte secretion of TNF-α and IL-6. (E and F) SA could active NF-κB, and the process could be inhibited by gAd. PBS and LPS were used as negative and positive control, respectively. Data are mean ± SEM. *P < 0.05 vs negative control and ^†P < 0.05 vs positive control. Triplicate experiments were performed with essentially identical results. Abbreviations: IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; SA, stearic acid; gAd, globular adiponectin; PBS, phosphate buffered saline; LPS, lipopolysaccharide.