Effects of nicorandil on p47phox and total eNOS, GCH-1 expressions and eNOS uncoupling in rat femoral artery. Nicorandil normalised increased p47phox protein (A) and total eNOS protein (B) in diabetic rat femoral arteries. Nicorandil also normalized decreased eNOS dimer/monomer ratio (C) and GCH-1 protein (D) in diabetic rat femoral arteries. Femoral arteries were harvested 4 weeks after STZ administration. *p < 0.05 vs normal, #p < 0.05 vs STZ (n = 6-8).