Figure 5
mRNA expression of angiotensin II type 1 receptor (C: AT 1 R, 352-bp fragment) and type 2 receptor (D: AT 2 R, 418-bp fragment) in aortic tissues. Representative samples of AT1R and AT2R mRNA from aortic samples in each group are shown at the top. The bar graph shows mRNA expression of AT1R or AT2R relative to internal control ß-actin (232-bp fragment) from 4 replicate experiments in each group. Con: the regular diet group; Cho: the cholesterol-rich diet plus methylthiouracil group; Ros: the group on cholesterol-rich diet plus methylthiouracil and rosiglitazone treatment. Note that the increase in AT1R mRNA caused by the cholesterol-rich diet plus methylthiouracil was markedly suppressed by rosiglitazone treatment (P < 0.01). However, mRNA expression of AT2R was further increased (P < 0.01). *P < 0.01 versus Con group; ‡P < 0.01 versus Cho group.