Diabetic retinopathy is associated with pulse wave velocity, not with the augmentation index of pulse waveform
© Ogawa et al; licensee BioMed Central Ltd. 2008
Received: 04 March 2008
Accepted: 25 April 2008
Published: 25 April 2008
To investigate the clinical differences between pulse wave velocity and augmentation index in diabetic retinopathy.
The subjects were 201 patients with type 2 diabetes. These subjects were measured for both augmentation index (AI) and brachial-ankle pulse wave velocity (baPWV) by a pulse wave analyzer. The relationships between AI, baPWV, and diabetic retinopathy were examined.
BaPWV was significantly higher in patients with diabetic retinopathy than in individuals without the disease. (20.13 ± 3.66 vs.17.14 ± 3.60 m/s p < 0.001) AI was higher in patients with diabetic retinopathy, but not significantly. (19.5 ± 15.2 vs. 14.8 ± 20.5% p = 0.14) The association between baPWV and diabetic retinopathy remained statistically significant after adjustment. (Odds ratio: 1.21 Per m/s, 95% confidence interval: 1.07–1.37) On the other hand, the association between AI and diabetic retinopathy was not statistically significant. (Odds ratio: 1.01 Per %, 95% confidence interval: 0.98–1.03)
BaPWV is associated with diabetic retinopathy, but AI is not. The clinical significance appears to be different between PWV and AI in patients with diabetes.
Both pulse wave velocity (PWV) and augmentation index (AI) are known as markers of arterial stiffness . These parameters have been associated with cardiovascular disease. The definition and measurement of these parameters is different, therefore, the clinical significance may also be different. PWV was defined as the speed with which the pulse wave travels along the length of an artery. Augmentation of the pulse waveform was defined as the difference between the second systolic peak (caused by wave reflection) and the first systolic peak (caused by left ventricular ejection). AI was this difference expressed as a percentage of the central pulse pressure. It is reported that type 2 diabetes is associated with increased AI, and decreased carotid-femoral transit time, which is related to PWV . On the other hand, it has been reported that there is no association between AI and PWV in patients with diabetes . Thus, the clinical difference between PWV and AI remains uncertain in patients with diabetes. We previously reported an association between diabetic retinopathy and brachial-ankle pulse wave velocity (baPWV) . However, we did not investigate the relationship between AI and diabetic retinopathy. In this study, to clarify the clinical difference between PWV and AI, we investigated the association between baPWV, AI and diabetic retinopathy.
Japanese patients with type 2 diabetes who had randomly undergone testing for both baPWV and AI of the common carotid artery between April 2002 and April 2003 at the outpatient department of the Juntendo University Hospital (Tokyo, Japan) were enrolled in this study. Informed consent was obtained from all subjects. Clinical data were collected in a cross sectional manner from medical records. Type 2 diabetes was indicated by a fasting plasma glucose concentration >7.0 mmol/L without treatment, a plasma glucose concentration 11.0 mmol/L measured 2 h after a 75 g oral glucose load, or by treatment with a glucose-lowering drug. The patients selected for the study were in a state of sinus rhythm. The patients selected for the study had no history of ketoacidosis, ischemic heart disease, peripheral artery disease or ischemic stroke. Patients with an ankle-brachial pressure index (ABI) <0.9 were excluded from the analysis. Diagnoses of diabetic retinopathy were made by ophthalmologists based on the presence of one or more of the following clinical features in the fundus: hemorrhages, hard or soft exudate, venous beading, intra-retinal microvascular abnormalities, cotton-wool spots, pre-retinal new vessels, fibrous proliferation and/or photocoagulation scars. Diabetic nephropathy was defined as a casual urinary albumin/creatinine ratio >30 mg/g Cr. Total cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride and hemoglobin A1c (HbA1c), urinary albumin and creatinine levels were determined using standard laboratory techniques.
Measurements of PWV and AI
Measurement of baPWV and AI were performed on the same day. The automatic pulse wave analyzer (Form/ABI with AT-unit; Omron Colin Co. Ltd, Tokyo) was used to measure baPWV, AI and ABI. This device has been approved by the US food and drug administration. The device simultaneously performs an electrocardiogram, phonocardiogram, bilateral brachial and ankle blood pressure (BP), and carotid arterial pulse wave. The detailed method to measure baPWV and ABI was previously reported [5, 6]. Briefly, measurement was performed with the patients in the supine position after resting for five minutes. Occlusion and monitoring cuffs were placed snugly around sites in the upper and lower extremities. Pressure waveforms were then recorded simultaneously from the brachial arteries by an oscillometric method. The ABI was calculated as follows: ABI = ankle systolic BP/brachial systolic BP. It is well known that patients with a low ABI exhibit a lower baPWV value than the actual value when assessed by the method described above . Thus, we excluded patients with an ABI level of less than 0.9 when evaluating baPWV.
Carotid arterial pulse wave were obtained using arterial applanation tonometry incorporating an array of 15 micropiezoresistive transducers. Advanced multiplexing electronics combined with automatic sensor selection relative to the target artery facilitated and ensured optional placement of the sensor. The carotid sensor was manually placed on the common carotid artery. The systolic portion of the central arterial waveform is characterized by two pressure peaks. The first peak is caused by left ventricular ejection, whereas the second peak is a result of wave reflection. The difference between both pressure peaks reflects the degree to which central arterial pressure is augmented by wave reflection. The AI (%) is defined as the percentage of the central pulse pressure which is represented by the reflected pulse wave and, therefore, reflects the degree to which central arterial pressure is augmented by wave reflection . The reliability and reproducibility of the AI measured by this device are reported to be very good [9, 10]. These measurements were taken with patients lying in a supine position after 5 min of rest.
In order to compare the association between baPWV and diabetic retinopathy, and between AI and diabetic retinopathy, we performed several statistical analyses. First, subjects were divided into two groups: those with and those without diabetic retinopathy. Clinical characteristics, including baPWV and AI, were analyzed within the two groups. Continuous variables were presented as the mean standard deviation (S.D.). Continuous variables were compared using Student's t-test and categorical data were compared using the chi-squared test. Second, we used linear regression analysis to investigate the correlation between baPWV and AI. Third, multiple regression analysis was performed to investigate the relationship between arterial stiffness markers and clinical parameters. Multiple logistic-regression analysis was used to provide the adjusted odds ratios (ORs) for baPWV and AI for diabetic retinopathy. The following factors were adjusted for: age, gender, duration of diabetes, HbA1c level, diabetic nephropathy, hypertension (systolic BP > 140 mmHg and/or diastolic BP > 90 mmHg) and hyperlipidemia (total cholesterol concentration > 5.7 mmol/L and/or triglyceride concentration >1.7 mmol/L and/or treatment with antihyperlipidemic agents). In addition, AI was increasing more in younger individuals [11, 12]. Therefore, the subjects were divided into two groups, under 60 years old and over 60 years old. We analyzed relationship between AI and retinopathy each groups. The ORs are presented with 95% confidence intervals (CIs). All statistical analyses were performed using StatView version 5.0 computer software (SAS Institute Inc. Cary, NC, USA). Probability values (p) < 0.05 were considered statistically significant.
Characteristics of the 201 patients enrolled in the study according to the presence (+) or absence (-) of diabetic retinopathy.
DR(+) n = 47
DR(-) n = 154
20.13 ± 3.66
17.14 ± 3.60
19.5 ± 15.2
14.8 ± 20.4
1.59 ± 0.08
1.63 ± 0.08
61.1 ± 10.6
59.7 ± 11.2
13.0 ± 8.5
8.6 ± 7.5
Systolic BP (mmHg)
142.7 ± 16.3
129.3 ± 17.9
Diastolic BP (mmHg)
82.8 ± 11.1
78.3 ± 9.56
Heart rate (beat/min)
72.4 ± 12.0
66.3 ± 10.5
7.2 ± 1.5
7.4 ± 1.9
Total cholesterol (mmol/L)
5.88 ± 2.74
5.14 ± 0.89
HDL cholesterol (mmol/L)
1.40 ± 0.47
1.31 ± 0.40
1.68 ± 0.88
1.74 ± 1.12
Antihypertensive agents (yes/no)
multiple regression analysis of baPWV and AI
Adjusted odds ratios for diabetic retinopathy
Adjusted odds ratio (95% CI)
Adjusted odds ratio (95% CI)
Duration of diabetes
In this study, there was a significant positive association between the presence of diabetic retinopathy and baPWV among Japanese type 2 diabetic patients without macroangiopathy. However, the association between AI and diabetic retinopathy was found to be uncertain. The correlation between baPWV and AI was weak. The determining factors were different between baPWV and AI.
Previously, we showed baPWV is associated with diabetic retinopathy . PWV is correlated with the duration of diabetes and with the accumulation of fluorescent advanced glycation end products in the aorta. The accumulation of advanced glycation end products has also been associated with the progression of diabetic retinopathy [14, 15]. Furthermore, the United Kingdom Prospective Diabetes Study (UKPDS) showed that the development of retinopathy was associated with long-term glycemic exposure [16, 17]. These findings indicate that the common mechanism of arterial stiffness and retinopathy might be the accumulation of advanced glycation end products due to chronic glycemic exposure. The result of the association of other factors and diabetic retinopathy found in this study was similar with that which we have previously reported. HbA1c and hypertension were not significantly associated with diabetic retinopathy. UKPDS showed the development of retinopathy to be associated with long-term glycemic exposure and hypertension [16–18]. The duration of diabetes was found to be associated with diabetic retinopathy in this study. However, HbA1c is a marker for short-term glycemic control (1 or 2 months) only; thus the effects of glycemic control on diabetic retinopathy might, therefore, not be evident in this study. In addition, BP was not measured before the onset of diabetic retinopathy in our study, so the effects of hypertension might not be apparent. These results could be a consequence of the cross-sectional study design.
The duration of diabetes determined baPWV, but not AI, in this study. In addition, PWV was increased in patients with diabetes, but this is not associated with AI . In this study, the correlation between baPWV and AI was weak. These results indicated that chronic hyperglycemia maynot be associated with increasing AI. On the other hand, diabetic retinopathy is associated with chronic hyperglycemia. Therefore, the result that AI was not associated with diabetic retinopathy in this study may be due to AI not being associated with the duration of diabetes.
AI was correlated with height, heart rate, SBP. On the other hand, baPWV was correlated with age, SBP, and heart rate . In addition, AI was influenced by vasoactive drugs independently of PWV . Thus, baPWV and AI were not interchangeable as markers of arterial stiffness. AI has stronger potential for use in endothelial function testing rather than as a marker of arterial stiffness compared with PWV . These differences between PWV and AI may have includend the results of the study.
Insulin sensitivity reportedly influences AI , but was not examined in this study. Differences in insulin sensitivity may influenced the result of this study. PWV and AI are both strongly related to age, but the age-related changes in PWV and AI are non linear, with AI increasing more in younger individuals [11, 12]. The age of the patients in study was not as advanced. These factors may be affected the result of our study.
A positive association between AI and diabetic retinopathy has been previously reported . In that study, subjects with macroangiopathy were included. Diabetic retinopathy is also reportedly associated with cardiovascular disease . Both AI and PWV are also associated with macroangiopathy [23, 24]. These associations may have influenced the reported result. In this study, to avoid the association between arterial stiffness and macroangiopathy, patients with macroangiopathy were excluded. In addition, data of height and heart rate were not included. Height and heart rate are reportedly associated with PWV and AI , so these unincorporated data may have influenced their result. Another possibility is that the devices in each study were different. A well-designed study will be needed to clearly elucidate the association between AI and diabetic retinopathy, and to identify the relevant features with utility for clinical practice.
PWV and AI are reported markers of arterial stiffness, but differing results have been obtained for diabetic retinopathy. The clinical significance of PWV and AI appears to be different in patients with diabetes.
The authors thank to Hirotaka Watada for helpful advice.
- Oliver JJ, Webb DJ: Noninvasive assessment of arterial stiffness and risk of atherosclerotic events. Arterioscler Thromb Vasc Biol. 2003, 23: 554-566. 10.1161/01.ATV.0000060460.52916.D6.View ArticlePubMedGoogle Scholar
- Schram MT, Henry RM, van Dijk RA, Kostense PJ, Dekker JM, Nijpels G, Heine RJ, Bouter LM, Westerhof N, Stehouwer CD: Increased central artery stiffness in impaired glucose metabolism and type 2 diabetes: the Hoorn Study. Hypertension. 2004, 43: 176-181. 10.1161/01.HYP.0000111829.46090.92.View ArticlePubMedGoogle Scholar
- Lacy PS, O'Brien DG, Stanley AG, Dewar MM, Swales PP, Williams B: Increased pulse wave velocity is not associated with elevated augmentation index in patients with diabetes. J Hypertens. 2004, 22: 1937-1944. 10.1097/00004872-200410000-00016.View ArticlePubMedGoogle Scholar
- Ogawa O, Hayashi C, Nakaniwa T, Tanaka Y, Kawamori R: Arterial stiffness is associated with diabetic retinopathy in type 2 diabetes. Diabetes Res Clin Pract. 2005, 68: 162-166. 10.1016/j.diabres.2004.09.008.View ArticlePubMedGoogle Scholar
- Yamashina A, Tomiyama H, Takeda K, Tsuda H, Arai T, Hirose K, Koji Y, Hori S, Yamamoto Y: Validity, reproducibility, and clinical significance of noninvasive brachial-ankle pulse wave velocity measurement. Hypertens Res. 2002, 25: 359-364. 10.1291/hypres.25.359.View ArticlePubMedGoogle Scholar
- Tomiyama H, Koji Y, Yambe M, Motobe K, Shiina K, Gulnisa Z, Yamamoto Y, Yamashina A: Elevated C-reactive protein augments increased arterial stiffness in subjects with the metabolic syndrome. Hypertension. 2005, 45: 997-1003. 10.1161/01.HYP.0000165018.63523.8a.View ArticlePubMedGoogle Scholar
- Suzuki E, Kashiwagi A, Nishio Y, Egawa K, Shimizu S, Maegawa H, Haneda M, Yasuda H, Morikawa S, Inubushi T, Kikkawa R: Increased arterial wall stiffness limits flow volume in the lower extremities in type 2 diabetic patients. Diabetes Care. 2001, 24: 2107-2114. 10.2337/diacare.24.12.2107.View ArticlePubMedGoogle Scholar
- O'Rourke MF, Gallagher DE: Pulse wave analysis. J Hypertens Suppl. 1996, 14: S147-57. 10.1097/00004872-199602000-00001.View ArticlePubMedGoogle Scholar
- Cortez-Cooper MY, Supak JA, Tanaka H: A new device for automatic measurements of arterial stiffness and ankle-brachial index. Am J Cardiol. 2003, 91: 1519-22, A9. 10.1016/S0002-9149(03)00416-8.View ArticlePubMedGoogle Scholar
- Matsui Y, Kario K, Ishikawa J, Eguchi K, Hoshide S, Shimada K: Reproducibility of arterial stiffness indices (pulse wave velocity and augmentation index) simultaneously assessed by automated pulse wave analysis and their associated risk factors in essential hypertensive patients. Hypertens Res. 2004, 27: 851-857. 10.1291/hypres.27.851.View ArticlePubMedGoogle Scholar
- McEniery CM, Yasmin, Hall IR, Qasem A, Wilkinson IB, Cockcroft JR: Normal vascular aging: differential effects on wave reflection and aortic pulse wave velocity: the Anglo-Cardiff Collaborative Trial (ACCT). J Am Coll Cardiol. 2005, 46: 1753-1760. 10.1016/j.jacc.2005.07.037.View ArticlePubMedGoogle Scholar
- Kohara K, Tabara Y, Oshiumi A, Miyawaki Y, Kobayashi T, Miki T: Radial augmentation index: a useful and easily obtainable parameter for vascular aging. Am J Hypertens. 2005, 18: 11S-14S. 10.1016/j.amjhyper.2004.10.010.View ArticlePubMedGoogle Scholar
- Airaksinen KE, Salmela PI, Linnaluoto MK, Ikaheimo MJ, Ahola K, Ryhanen LJ: Diminished arterial elasticity in diabetes: association with fluorescent advanced glycosylation end products in collagen. Cardiovasc Res. 1993, 27: 942-945. 10.1093/cvr/27.6.942.View ArticlePubMedGoogle Scholar
- Murata T, Nagai R, Ishibashi T, Inomuta H, Ikeda K, Horiuchi S: The relationship between accumulation of advanced glycation end products and expression of vascular endothelial growth factor in human diabetic retinas. Diabetologia. 1997, 40: 764-769. 10.1007/s001250050747.View ArticlePubMedGoogle Scholar
- Singh R, Barden A, Mori T, Beilin L: Advanced glycation end-products: a review. Diabetologia. 2001, 44: 129-146. 10.1007/s001250051591.View ArticlePubMedGoogle Scholar
- Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33). UK Prospective Diabetes Study (UKPDS) Group. Lancet. 1998, 352: 837-853. 10.1016/S0140-6736(98)07019-6.
- Stratton IM, Kohner EM, Aldington SJ, Turner RC, Holman RR, Manley SE, Matthews DR: UKPDS 50: risk factors for incidence and progression of retinopathy in Type II diabetes over 6 years from diagnosis. Diabetologia. 2001, 44: 156-163. 10.1007/s001250051594.View ArticlePubMedGoogle Scholar
- Tight blood pressure control and risk of macrovascular and microvascular complications in type 2 diabetes: UKPDS 38. UK Prospective Diabetes Study Group. Bmj. 1998, 317: 703-713.
- Kelly RP, Millasseau SC, Ritter JM, Chowienczyk PJ: Vasoactive drugs influence aortic augmentation index independently of pulse-wave velocity in healthy men. Hypertension. 2001, 37: 1429-1433.View ArticlePubMedGoogle Scholar
- Tamminen M, Westerbacka J, Vehkavaara S, Yki-Jarvinen H: Insulin-induced decreases in aortic wave reflection and central systolic pressure are impaired in type 2 diabetes. Diabetes Care. 2002, 25: 2314-2319. 10.2337/diacare.25.12.2314.View ArticlePubMedGoogle Scholar
- Rema M, Mohan V, Deepa R, Ravikumar R: Association of carotid intima-media thickness and arterial stiffness with diabetic retinopathy: the Chennai Urban Rural Epidemiology Study (CURES-2). Diabetes Care. 2004, 27: 1962-1967. 10.2337/diacare.27.8.1962.View ArticlePubMedGoogle Scholar
- Juutilainen A, Lehto S, Ronnemaa T, Pyorala K, Laakso M: Retinopathy predicts cardiovascular mortality in type 2 diabetic men and women. Diabetes Care. 2007, 30: 292-299. 10.2337/dc06-1747.View ArticlePubMedGoogle Scholar
- Weber T, Auer J, O'Rourke MF, Kvas E, Lassnig E, Berent R, Eber B: Arterial stiffness, wave reflections, and the risk of coronary artery disease. Circulation. 2004, 109: 184-189. 10.1161/01.CIR.0000105767.94169.E3.View ArticlePubMedGoogle Scholar
- Ogawa O, Onuma T, Kubo S, Mitsuhashi N, Muramatsu C, Kawamori R: Brachial-ankle pulse wave velocity and symptomatic cerebral infarction in patients with type 2 diabetes: a cross-sectional study. Cardiovasc Diabetol. 2003, 2: 10-10.1186/1475-2840-2-10.PubMed CentralView ArticlePubMedGoogle Scholar
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