Inflammatory biomarkers in type 2 diabetic patients: effect of glycemic control and impact of ldl subfraction phenotype
© Vinagre et al.; licensee BioMed Central Ltd. 2014
Received: 9 January 2014
Accepted: 3 February 2014
Published: 4 February 2014
Type 2 diabetes mellitus (T2D) is associated with higher cardiovascular risk partly related to an increase in inflammatory parameters. The aim of this study was to determine the association of inflammatory biomarkers with low-density lipoprotein (LDL) subfraction phenotype and glycemic control in subjects with T2D and poor glycemic control.
A cross-sectional study was performed comparing 122 subjects with T2D (59 ± 11 years old, body mass index 30.2 ± 5.6 kg/m2) with 54 control subjects. Patients with T2D were classified according to their LDL subfraction phenotype and inflammatory biomarkers (C-reactive protein, Interleukin-6, Interleukin-8, Transforming growth factor β1, Monocyte chemotactic protein 1, Leptin, Adiponectin) were evaluated according to the degree of glycemic control, LDL phenotype and other clinical characteristics. Forty-two subjects with T2D were studied before and after 3 months of improving glycemic control by different strategies.
Patients with T2D had higher C-reactive protein (CRP) and monocyte chemotactic protein-1 (MCP1) levels and lower adiponectin concentration, compared to controls. T2D subjects with body mass index ≥ 30 kg/m2 had higher CRP levels (5.2 ± 4.8 mg/l vs 3.7 ± 4.3 mg/l; p < 0.05). The presence of LDL phenotype B was related to higher levels of transforming growth factor-β1 (TGF-β1) (53.92 ± 52.82 ng/l vs 31.35 ± 33.74 ng/l; p < 0.05) and lower levels of adiponectin (3663 ± 3044 ng/l vs 2723 ± 1776 ng/l; p < 0.05). The reduction of HbA1c from 9.5 ± 1.8% at baseline to 7.4 ± 0.8% was associated with a significant reduction of TGF-β1 (41.86 ± 32.84 ng/l vs 26.64 ± 26.91 ng/l; p = 0.02).
Subjects with T2D, especially those with LDL phenotype B and obesity, have higher levels of inflammatory biomarkers. Improvement of glycemic control reduces TGF-β1 levels, which may contribute partly to its renoprotective role.
KeywordsInflammatory biomarkers C-reactive protein Adiponectin Monocyte chemotactic protein-1 Transforming growth factor-β1 Tumor growth factor β1 Type 2 diabetes LDL phenotype Atherogenic dyslipidemia
Type 2 diabetes mellitus is one of the most common diseases in occidental society and it is associated with a high cardiovascular risk, not only due to the classical factors but also to a chronic low-grade inflammation . In this sense, epidemiological studies have demonstrated an increase in plasma levels of inflammatory markers such as CRP, IL-6 and TNF-α in patients with metabolic syndrome and also in those with clinically overt T2D [2, 3]. Other molecules such as the transforming growth factor (or tumor growth factor) β1, MCP1 [5, 6] or LP-PLA2  also present increased concentrations in T2D subjects.
A genetic predisposition associated to excessive caloric intake and a lack of physical excercise can lead to obesity and central adiposity. Then, this may result in adipose tissue dysfunction, macrophage infiltration and a greater release of cytokines such as IL-6 and TNF-α. Chronically elevated levels of these biomarkers promote insulinresistance in skeletal muscle and endothelial dysfunction, as well as liberation of CRP from the liver . Moreover, hyperglycaemia also induces IL-6 production from endothelium and macrophages, which might worsen insulin liberation and signaling cascades. This suggests that improving glycemic control might reduce the inflammatory response supporting the link between inflammation and glucose metabolic disturbance.
On the other hand, previous studies have demonstrated a significant correlation between CRP levels and features of the metabolic syndrome, including adiposity, hyperinsulinemia, insulin resistance, hypertriglyceridemia and low HDLc [9–11]. Interestingly, lifestyle and pharmacological interventions in T2D such as statins, angiotensin receptor blockers and glitazones have demonstrated a beneficial effect on markers of inflammation [12–15]. However, the correlation between inflammatory markers and glycemic control is significant in some studies [16, 17] but not in others , and available information about the effect of an improvement of glycemic control on inflammatory markers is very scarce [19, 20]. Moreover, the impact of the presence of the atherogenic LDL subfraction phenotype, very common in T2D, on inflammatory markers has not been previously analyzed in these subjects.
Therefore, the aim of this study was to determine the association of inflammatory biomarkers with glycemic control and LDL subfraction phenotype.
Anthropometric and clinical characteristics of control subjects and patients with type 2 diabetes at baseline
Control group (N = 54)
Type 2 diabetes (N = 122)
56 ± 14
59 ± 11
Diabetes duration (years)
10.61 ± 10.26
● Retinopathy (%)
● Nephropathy (%)a
● Coronary Heart Disease (%)
Antihypertensive medication (%)
Cholesterol lowering medication (%)
69.3 ± 11.4
82.1 ± 18.8*
92 ± 11
105 ± 14*
Body mass index (Kg/m2)
25.9 ± 3.5
30.2 ± 5.6*
The study was performed in accordance with the principles of the Declaration of Helsinki and was approved by the Hospital’s ethics committee. All patients and controls signed informed consent.
Blood specimens were obtained after overnight fasting. HbA1c was measured by ion-exchange high-performance liquid chromatography (HPLC; variant II, Bio-Rad) with a reference range of 4.6-5.8%. LDL subfraction phenotype was determined by measuring LDL size, determined by non-denaturing polyacrylamide gradient (2–16%) gel electrophoresis, as described . LDL subfraction phenotype B was defined by a predominant LDL diameter lower than 25.5 nm, while phenotype A subjects had a LDL diameter higher than 25.5 nm. C-reactive protein was measured by a highly-sensitive commercial method (hsCRP, Roche Diagnostics) in Hitachi 917 autoanalyzer. Interleukin 6, IL-8, MCP1, TGF-β1, leptin and adiponectin were measured by ELISA from Bender Medsystems (IL-6, IL-8, MCP1 and TGF-β1), or R&D (leptin and adiponectin).
Statistical analysis was performed using SPSS version 15.0 (SPSS Inc.). Before statistical analysis, normal distribution and homogeneity of the variances were tested using Kolmogorov-Smirnov and Levène tests, respectively. Data that were not normally distributed were logarithmically transformed before analysis. Groups were compared using Student’s unpaired t test for parameters with normal distribution or Mann–Whitney test for parameters with non-normal distribution. The effect of glycemic optimization was analyzed using Student’s paired t test for parameters with normal distribution or Wilcoxon test for parameters with non-normal distribution. Correlations between parameters were analyzed using the Pearson R test for variables with normal distribution and the Spearman test for variables with non-normal distribution. Data are expressed as mean ± standard desviation. P < 0.05 was considered significant.
Inflammation parameters of control subjects and patients with type 2 diabetes mellitus, according LDL subfraction phenotype, at baseline: T2D: type 2 diabetes
T2D (N = 122)
T2D phenotype A (N = 77)
T2D phenotype B (N = 45)
Control group (N = 54)
C-reactive protein (mg/l)
4.49 ± 4.67 a
4.31 ± 4.52
4.86 ± 5.10
2.38 ± 3.30
1.84 ± 3.99
2.24 ± 4.95
1.17 ± 0.83
1.25 ± 1.05
15.91 ± 12.85
17.46 ± 13.74
13.20 ± 10.87
12.58 ± 9.29
Transforming growth factor β1 (ng/l)
41.19 ± 30.08
31.35 ± 33.74
53.92 ± 52.82 b
39.55 ± 42.86
Monocyte chemotactic protein 1 (ng/l)
198.0 ± 132.9 a
190.6 ± 125.6
212.1 ± 146.21
154.3 ± 83.0
8980 ± 8194
9066 ± 8786
8787 ± 7184
7819 ± 5866
3272.0 ± 2671 a
3663 ± 3044
2723 ± 1776 b
5369 ± 3297
Clinical characteristics and inflammation parameters of patients with type 2 diabetes mellitus before and after glycemic optimization (n = 42)
After glycemic optimization
61 ± 12
Diabetes duration (years)
37 ± 14
● Retinopathy (%)
● Nephropathy (%)
● Coronary Heart Disease (%)
Insulin use (%)
Cholesterol lowering medication (%)
80.35 ± 19.52
80.02 ± 19.71
Body mass index (Kg/m2)
29.0 ± 5.8
28.8 ± 6
9.5 ± 1.8
7.4 ± 0.8*
C-reactive protein (mg/l)
3.20 ± 2.86
3.15 ± 3.26
1.49 ± 1.12
1.29 ± 0.70
12.84 ± 7.57
13.82 ± 10.35
Transforming growth factor β1 (ng/l)
41.86 ± 32.84
26.64 ± 26.91*
Monocyte chemotactic protein 1 (ng/l)
224.31 ± 76.03
227.48 ± 132.79
7594 ± 8852
7817 ± 7295
3673 ± 3455
4084 ± 3927
The present study confirms the low grade systemic inflammation status in T2D patients which is related to features of the metabolic syndrome. However, the main and novel findings are related to increased levels of TGF-β1 concentration in patients with LDL phenotype B, compared to those with phenotype A, and its reduction after a marked improvement of glycemic control. These findings suggest that TGF-β1 may be a link to glycemic control and atherogenic dyslipidemia with the development of diabetic nephropathy.
Systemic inflammation status in T2D subjects
The analysis of inflammatory parameters showed that plasma concentrations of CRP and MCP1 were higher and that of adiponectin was lower in T2D patients than in control subjects. This observation indicates a greater global systemic inflammation status in diabetic patients, which has been previously suggested that may reflect the activity of the underlying atherosclerotic process , and it is concordant with several cross-sectional studies which have shown an increase of CRP levels in patients with diabetes [25–27]. Although the results reported in the literature on the association between IL-6 and T2D are contradictory [26, 28, 29], the absence of statistical differences in IL-6 and other inflammatory parameters could be related to the fact that most patients were treated with medications such as metformin, antihypertensive agents and lipid-lowering drugs, which have demonstrated a beneficial effect on inflammation [12–14]. In addition, the available information about the relationship between inflammatory biomarkers and the characteristics of the diabetes is limited. Some studies have demonstrated significant correlation between CRP levels with features of the metabolic syndrome, including adiposity, hyperinsulinemia, insulin resistance, hypertriglyceridemia and low HDLc [9, 30]. The preponderance of small LDL particles (LDL phenotype B) is a component of atherogenic dyslipidemia. In accordance, we have observed that obese T2D subjects have higher CRP concentrations and inflammatory status was more unfavorable in patients with LDL phenotype B, who had higher concentration of TGF-β1 and lower of adiponectin than patients with phenotype A. To our knowledge, no other studies have examined this association, but there are some publications agreeing that serum levels of TGF-β1 are higher in patients with metabolic syndrome than those without it  and that oxidized low-density lipoprotein, which is increased in subjects with LDL phenotype B, is associated with TGF-β1 in T2D subjects . Finally, TGFβ family members have been involved in vascular remodeling defects in experimental models , which could contribute to explain the worse prognostic of atherosclerotic cardiovascular disease in diabetic patients.
The effect of improving glycemic control on markers of inflammation in T2D
It has been shown that in the individuals with impaired glucose tolerance  the low-grade chronic inflammation is related to glucose metabolic disturbance and a growing body of evidence supports the hypothesis that chronic systemic inflammation contributes to decrease insulin sensitivity . Moreover, hyperglycemia is a significant stressor that has also been shown to cause chronic inflammation . However, the relationship between inflammatory markers and glycemic control is still not fully understood. In our study, no inflammation marker correlated with HbA1c levels. These results are consistent with the findings of previous cross-sectional studies which have found an inconsistent association between inflammation and blood glucose levels [16–18], possibly due to previous treatment with metformin, statins and antihypertensive agents, which may limit the power to detect an association between HbA1c and inflammatory markers. In the longitudinal study, we demonstrated for the first time that improvement of glycemic control by different therapeutic strategies can lower TGF-β1 concentrations. These results are consistent with the findings in diabetic rats treated with insulin and with the increased expression of TGF-β1 receptor in the diabetic kidney and in mesangial cells cultured in high glucose [37, 38]. The lack of significant correlation between the magnitude of the reductions in HbA1c and TGF-β1 after glycemic optimization may reflect differences in the relative importance of the local versus systemic blood glucose levels, and it does not preclude an underlying relationship between these two parameters. The lack of change in CRP and other inflammatory markers in our study cannot be attributed to weight gain or pharmacological therapy, and confirms the results in the few studies which have evaluated the effect of improving glycemic control on markers of inflammation in type 1 diabetes (DCCT) and T2D [19, 20]. In the DCCT study, intensive glycemic control was not associated with changes in levels of CRP and among intensively treated subjects who gained the most weight, there was a significant rise. In T2D, in a study of 18 patients who had been hospitalized to initiate insulin therapy because of poor diabetes control, CRP values, but not MCP1 and fibrinogen levels, were decreased 2 weeks after initiation of insulin therapy. This effect could result from anti-inflammatory effects of insulin , although these findings were not confirmed in the study of Pradhan et al. . They found that, in patients with recent-onset T2D, treatment during 14 weeks with insulin glargine or metformin compared with placebo did not reduce CRP, IL-6 and soluble tumor necrosis factor receptor 2, despite improving glucose control. In the present study, the duration of the study was sufficient on the basis of assessment of glycemic control by HbA1c, and a marked improvement of glycemic control was obtained, being nearly normal in most of the patients without changes in body weight. Furthermore, lipid-lowering and antihypertensive therapy was unchanged and in most patients insulin therapy was the strategy used to improve glycemic control.
The possible role of TGF-β1 in diabetic nephropathy
The critical role of hyperglycemia in the genesis of diabetic nephropathy has been established by cell culture studies, experimental animal models, and clinical trials. Certain cytokines have been identified as likely mediators of the effects of high ambient glucose on the kidney. TGF-β1, a known pro-fibrotic factor, activates the production of extracellular matrix by mesangial cells and interstitial fibroblasts in the kidneys, and thus contributes to the manifestation of diabetic kidney disease through a number of key pathological events leading to reduced glomerular filtration and impaired renal function [39, 40]. In mesangial cells, it has been demonstrated that the expression and activity of TGF-β1 are directly influenced by glucose levels . Moreover, atherogenic dyslipidemia is associated with the onset and progression of nephropathy in T2D subjects, by a mechanism not fully understood [41, 42]. Therefore, the finding of a higher concentration of TGF-β1 in patients with atherogenic dyslipidemia provides a potential explanation.
In conclusion, the present study confirms that low-grade chronic inflammation in T2D is related to LDL phenotype B and obesity. In addition, our data demonstrates that improvement of glycemic control reduces TGF-β1 levels, through an unknown mechanism. These findings might support the role of TGF-β1 as a likely mediator of the effects of high ambient glucose on the kidney and the beneficial effects of strict glucose control on the development of diabetic nephropathy. However, future studies in larger groups of patients should be performed to confirm these findings.
Type 2 diabetes mellitus
Tumor necrosis factor
Chemotactic monocyte protein 1
Lipoprotein-associated phospholipase A2
Low density lipoproteins cholesterol
High density lipoproteins cholesterol
Transforming growth factor or tumor growth factor β1
Body mass index.
This work was supported by grants from Spanish Ministry of Health (ISCIII/FIS) PI05/2099, PI05/1540, PI06/0500, and PI10/00265. JLS-Q is supported by ISCIII/FIS CP06/0220. JLS-Q and JO-L are members of the Quality Research Group 2009-SGR-1205 from Generalitat de Catalunya. AP and ADL are members of the Quality Research Group 2009-SGR-806 from Generalitat de Catalunya. J.S-H, D.S. and A.P. are members of CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) from ISCIII. JLS-Q and JO-L are members of the Cardiovascular Research Network (RIC, RD12/0042/0043) from ISCIII. The authors are grateful to Melissa Vance for editorial assistance.
- Eschwege E: The dysmetabolic syndrome, insulin resistance and increased cardiovascular (CV) morbidity and mortality in type 2 diabetes: aetiological factors in the development of CV complications. Diabetes Metab. 2003, 29 (4 Pt 2): 6S19-6S27.PubMed
- Pickup JC, Chusney GD, Thomas SM, Burt D: Plasma interleukin-6, tumour necrosis factor alpha and blood cytokine production in type 2 diabetes. Life Sci. 2000, 67 (3): 291-300. 10.1016/S0024-3205(00)00622-6.View ArticlePubMed
- Mirza S, Hossain M, Mathews C, Martinez P, Pino P, Gay JL, Rentfro A, McCormick JB, Fisher-Hoch SP: Type 2-diabetes is associated with elevated levels of TNF-alpha, IL-6 and adiponectin and low levels of leptin in a population of Mexican Americans: a cross-sectional study. Cytokine+. 2012, 57 (1): 136-142.PubMed CentralPubMed
- Azar ST, Salti I, Zantout MS, Major S: Alterations in plasma transforming growth factor beta in normoalbuminuric type 1 and type 2 diabetic patients. J Clin Endocrinol Metab. 2000, 85 (12): 4680-4682.PubMed
- Piemonti L, Calori G, Mercalli A, Lattuada G, Monti P, Garancini MP, Costantino F, Ruotolo G, Luzi L, Perseghin G: Fasting plasma leptin, tumor necrosis factor-alpha receptor 2, and monocyte chemoattracting protein 1 concentration in a population of glucose-tolerant and glucose-intolerant women: impact on cardiovascular mortality. Diabetes Care. 2003, 26 (10): 2883-2889. 10.2337/diacare.26.10.2883.View ArticlePubMed
- Sell H, Eckel J: Monocyte chemotactic protein-1 and its role in insulin resistance. Curr Opin Lipidol. 2007, 18 (3): 258-262. 10.1097/MOL.0b013e3281338546.View ArticlePubMed
- Serban M, Tanaseanu C, Kosaka T, Vidulescu C, Stoian I, Marta DS, Tanaseanu S, Moldoveanu E: Significance of platelet-activating factor acetylhydrolase in patients with non-insulin-dependent (type 2) diabetes mellitus. J Cell Mol Med. 2002, 6 (4): 643-647. 10.1111/j.1582-4934.2002.tb00462.x.View ArticlePubMed
- Calle MC, Fernandez ML: Inflammation and type 2 diabetes. Diabetes Metab. 2012, 38 (3): 183-91. 10.1016/j.diabet.2011.11.006.View ArticlePubMed
- Festa A, D’Agostino R, Howard G, Mykkanen L, Tracy RP, Haffner SM: Chronic subclinical inflammation as part of the insulin resistance syndrome: the Insulin Resistance Atherosclerosis Study (IRAS). Circulation. 2000, 102 (1): 42-47. 10.1161/01.CIR.102.1.42.View ArticlePubMed
- Lee CC, Adler AI, Sandhu MS, Sharp SJ, Forouhi NG, Erqou S, Luben R, Bingham S, Khaw KT, Wareham NJ: Association of C-reactive protein with type 2 diabetes: prospective analysis and meta-analysis. Diabetologia. 2009, 52 (6): 1040-1047. 10.1007/s00125-009-1338-3.View ArticlePubMed
- Nakano S, Kuboki K, Matsumoto T, Nishimura C, Yoshino G: Small, dense LDL and high-sensitivity C-reactive protein (hs-CRP) in metabolic syndrome with type 2 diabetes mellitus. J Atheroscler Thromb. 2010, 17 (4): 410-415. 10.5551/jat.1891.View ArticlePubMed
- Suzuki H, Sakamoto M, Hayashi T, Iuchi H, Ohashi K, Isaka T, Sakamoto N, Kayama Y, Tojo K, Yoshimura M, Utsunomiya K: Effects of co-administration of candesartan with pioglitazone on inflammatory parameters in hypertensive patients with type 2 diabetes mellitus: a preliminary report. Cardiovasc Diabetol. 2013, 12: 71-10.1186/1475-2840-12-71.PubMed CentralView ArticlePubMed
- Tan KC, Chow WS, Tam SC, Ai VH, Lam CH, Lam KS: Atorvastatin lowers C-reactive protein and improves endothelium-dependent vasodilation in type 2 diabetes mellitus. J Clin Endocrinol Metab. 2002, 87 (2): 563-568. 10.1210/jcem.87.2.8249.View ArticlePubMed
- Wagner AM, Sanchez-Quesada JL, Benitez S, Bancells C, Ordonez-Llanos J, Perez A: Effect of statin and fibrate treatment on inflammation in type 2 diabetes. A randomized, cross-over study. Diabetes Res Clin Pract. 2011, 93 (1): e25-e28. 10.1016/j.diabres.2011.03.009.View ArticlePubMed
- Belalcazar LM: A 1-year lifestyle intervention for weight loss in individuals with type 2 diabetes reduces high C-reactive protein levels and identifies metabolic predictors of change: from the Look AHEAD (Action for Health in Diabetes) study. Diabetes Care. 2010, 33 (11): 2297-303. 10.2337/dc10-0728.PubMed CentralView ArticlePubMed
- Leinonen E, Hurt-Camejo E, Wiklund O, Hulten LM, Hiukka A, Taskinen MR: Insulin resistance and adiposity correlate with acute-phase reaction and soluble cell adhesion molecules in type 2 diabetes. Atherosclerosis. 2003, 166 (2): 387-394. 10.1016/S0021-9150(02)00371-4.View ArticlePubMed
- Rodriguez-Moran M, Guerrero-Romero F: Increased levels of C-reactive protein in noncontrolled type II diabetic subjects. J Diabetes Complications. 1999, 13 (4): 211-215. 10.1016/S1056-8727(99)00047-1.View ArticlePubMed
- Pickup JC, Mattock MB, Crook MA, Chusney GD, Burt D, Fitzgerald AP: Serum sialic acid concentration and coronary heart disease in NIDDM. Diabetes Care. 1995, 18 (8): 1100-1103. 10.2337/diacare.18.8.1100.View ArticlePubMed
- Pradhan AD, Everett BM, Cook NR, Rifai N, Ridker PM: Effects of initiating insulin and metformin on glycemic control and inflammatory biomarkers among patients with type 2 diabetes: the LANCET randomized trial. JAMA. 2009, 302 (11): 1186-1194. 10.1001/jama.2009.1347.View ArticlePubMed
- Takebayashi K, Aso Y, Inukai T: Initiation of insulin therapy reduces serum concentrations of high-sensitivity C-reactive protein in patients with type 2 diabetes. Methods. 2004, 53 (6): 693-699.
- Sanchez-Quesada JL, Vinagre I, Juan-Franco E, Sanchez-Hernandez J, Blanco-Vaca F, Ordonez-Llanos J, Perez A: Effect of improving glycemic control in patients with type 2 diabetes mellitus on low-density lipoprotein size, electronegative low-density lipoprotein and lipoprotein-associated phospholipase A2 distribution. Am J Cardiol. 2012, 110 (1): 67-71. 10.1016/j.amjcard.2012.02.051.View ArticlePubMed
- Sanchez-Quesada JL, Vinagre I, de Juan-Franco E, Sanchez-Hernandez J, Bonet-Marques R, Blanco-Vaca F, Ordonez-Llanos J, Perez A: Impact of the LDL subfraction phenotype on Lp-PLA2 distribution, LDL modification and HDL composition in type 2 diabetes. Cardiovasc Diabetol. 2013, 12: 112-10.1186/1475-2840-12-112.PubMed CentralView ArticlePubMed
- Sanchez-Quesada JL, Benitez S, Otal C, Franco M, Blanco-Vaca F, Ordonez-Llanos J: Density distribution of electronegative LDL in normolipemic and hyperlipemic subjects. J Lipid Res. 2002, 43 (5): 699-705.PubMed
- Baars T, Konorza T, Kahlert P, Mohlenkamp S, Erbel R, Heusch G, Kleinbongard P: Coronary aspirate TNFalpha reflects saphenous vein bypass graft restenosis risk in diabetic patients. Cardiovasc Diabetol. 2013, 12: 12-10.1186/1475-2840-12-12.PubMed CentralView ArticlePubMed
- Wannamethee SG, Lowe GD, Rumley A, Cherry L, Whincup PH, Sattar N: Adipokines and risk of type 2 diabetes in older men. Diabetes Care. 2007, 30 (5): 1200-1205. 10.2337/dc06-2416.View ArticlePubMed
- Wang X, Bao W, Liu J, Ouyang YY, Wang D, Rong S, Xiao X, Shan ZL, Zhang Y, Yao P, Liu LG: Inflammatory Markers and Risk of Type 2 Diabetes: A systematic review and meta-analysis. Diabetes Care. 2013, 36 (1): 166-175. 10.2337/dc12-0702.PubMed CentralView ArticlePubMed
- Festa A, D’Agostino R, Tracy RP, Haffner SM: Elevated levels of acute-phase proteins and plasminogen activator inhibitor-1 predict the development of type 2 diabetes: the insulin resistance atherosclerosis study. Diabetes. 2002, 51 (4): 1131-1137. 10.2337/diabetes.51.4.1131.View ArticlePubMed
- Bertoni AG, Burke GL, Owusu JA, Carnethon MR, Vaidya D, Barr RG, Jenny NS, Ouyang P, Rotter JI: Inflammation and the incidence of type 2 diabetes: the Multi-Ethnic Study of Atherosclerosis (MESA). Diabetes Care. 2010, 33 (4): 804-810. 10.2337/dc09-1679.PubMed CentralView ArticlePubMed
- Krakoff J, Funahashi T, Stehouwer CD, Schalkwijk CG, Tanaka S, Matsuzawa Y, Kobes S, Tataranni PA, Hanson RL, Knowler WC, Lindsay RS: Inflammatory markers, adiponectin, and risk of type 2 diabetes in the Pima Indian. Diabetes Care. 2003, 26 (6): 1745-1751. 10.2337/diacare.26.6.1745.View ArticlePubMed
- Yudkin JS, Stehouwer CD, Emeis JJ, Coppack SW: C-reactive protein in healthy subjects: associations with obesity, insulin resistance, and endothelial dysfunction: a potential role for cytokines originating from adipose tissue?. Arterioscler, Thromb, Vasc Biol. 1999, 19 (4): 972-978. 10.1161/01.ATV.19.4.972.View Article
- Sciarretta S, Ferrucci A, Ciavarella GM, De PP, Venturelli V, Tocci G, De BL, Rubattu S, Volpe M: Markers of inflammation and fibrosis are related to cardiovascular damage in hypertensive patients with metabolic syndrome. Am J Hypertens. 2007, 20 (7): 784-791. 10.1016/j.amjhyper.2007.01.023.View ArticlePubMed
- Nakhjavani M, Esteghamati A, Asgarani F, Khalilzadeh O, Nikzamir A, Safari R: Association of oxidized low-density lipoprotein and transforming growth factor-beta in type 2 diabetic patients: a cross-sectional study. Transl Res. 2009, 153 (2): 86-90. 10.1016/j.trsl.2008.11.009.View ArticlePubMed
- Di BE, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q: Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways. J Biol Chem. 2013, [Epub ahead of print]
- Muller S, Martin S, Koenig W, Hanifi-Moghaddam P, Rathmann W, Haastert B, Giani G, Illig T, Thorand B, Kolb H: Impaired glucose tolerance is associated with increased serum concentrations of interleukin 6 and co-regulated acute-phase proteins but not TNF-alpha or its receptors. Diabetologia. 2002, 45 (6): 805-812. 10.1007/s00125-002-0829-2.View ArticlePubMed
- Pickup JC: Inflammation and activated innate immunity in the pathogenesis of type 2 diabetes. Diabetes Care. 2004, 27 (3): 813-823. 10.2337/diacare.27.3.813.View ArticlePubMed
- Astrup A, Finer N: Redefining type 2 diabetes: ‘diabesity’ or ‘obesity dependent diabetes mellitus’?. Obes Rev. 2000, 1 (2): 57-59. 10.1046/j.1467-789x.2000.00013.x.View ArticlePubMed
- Isono M, Mogyorosi A, Han DC, Hoffman BB, Ziyadeh FN: Stimulation of TGF-beta type II receptor by high glucose in mouse mesangial cells and in diabetic kidney. Am J Physiol Renal Physiol. 2000, 278 (5): F830-F838.PubMed
- Koppel H, Riedl E, Braunagel M, Sauerhoefer S, Ehnert S, Godoy P, Sternik P, Dooley S, Yard BA: L-carnosine inhibits high-glucose-mediated matrix accumulation in human mesangial cells by interfering with TGF-beta production and signalling. Nephrol Dial Transplant. 2011, 26 (12): 3852-3858. 10.1093/ndt/gfr324.View ArticlePubMed
- Lim AK, Tesch GH: Inflammation in diabetic nephropathy. Mediators Inflamm. 2012, 2012: 146154.PubMed CentralView ArticlePubMed
- Loeffler I, Wolf G: Transforming growth factor-beta and the progression of renal disease. Nephrol Dial Transplant. 2013, 2013: 2013.
- Caramori ML, Fioretto P, Mauer M: The need for early predictors of diabetic nephropathy risk: is albumin excretion rate sufficient?. Diabetes. 2000, 49 (9): 1399-1408. 10.2337/diabetes.49.9.1399.View ArticlePubMed
- Muntner P, Coresh J, Smith JC, Eckfeldt J, Klag MJ: Plasma lipids and risk of developing renal dysfunction: the atherosclerosis risk in communities study. Kidney Int. 2000, 58 (1): 293-301. 10.1046/j.1523-1755.2000.00165.x.View ArticlePubMed
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